Rapid identification of Staphylococcus aureus and methicillin resistance by flow cytometry using a peptide nucleic acid probe.

نویسندگان

  • Nabin K Shrestha
  • Nikole M Scalera
  • Deborah A Wilson
  • Byron Brehm-Stecher
  • Gary W Procop
چکیده

A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 49 9  شماره 

صفحات  -

تاریخ انتشار 2011